Figure 4.

Mice with a Knockin Mutation in the UBA Are Acutely Sensitive to TNF-Induced Systemic Inflammatory Response Syndrome

(A and B) Body temperature and survival of WT (A, n = 11; B, n = 12) and corresponding littermate cIAP1^UBAmut (A, n = 8) or cIAP2^−/− (B, n = 11) mice injected with 4 μg/20 g body weight of mTNF. Data are representative of two independent experiments. Error bars represent SD. Survival curves were compared using log-rank Mantel-Cox test (^∗p < 0.05, ^∗∗∗p < 0.001).

(C and D) Plasma samples of WT and cIAP1^UBAmut (C) or cIAP2^−/− (D) mice were collected at the indicated time points following challenge with mTNF (4 μg/20 g body weight, intravenously [i.v.]) and analyzed for activities of LDH, AST, and ALT. n = 4 per time point and genotype. Data are presented as mean ± SD, ^∗∗p < 0.01; statistics were performed using two-way ANOVA.

(E and F) TUNEL staining (E) and quantification (F) of liver sections of WT and cIAP1^UBAmut mice used in (C) and (D).

Data in (A) and (B) were obtained from two sets of animals, while the data shown in (C)–(F) were obtained from a third set of animals. Graphs show mean ± SD, ^∗∗p < 0.01; statistics were performed using two-way ANOVA.