Figure 6.

The UBA Directly Regulates RIPK1 Ubiquitylation

(A and B) Purification of the TNF-R1 signaling complex (complex-I) from primary MEFs (A) and MDFs (B). Cells of the indicated genotypes were treated with FLAG-TNF for 0, 5, and 60 min. Cell lysates were then subjected to FLAG IP followed by western blot analysis with the indicated antibodies. Representative images of three independent experiments are shown.

(C) Purification of the TNF-R1 signaling complex (complex-I) from immortalized cIAP2^−/− and cIAP1^UBAmut MEFs reconstituted either with empty vector (control) or a doxycycline-inducible construct encoding cIAP1^UBAmut.