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. 2018 Jan 29;41(2):119–126. doi: 10.14348/molcells.2018.2235

Fig. 4. miR-612 targets Espin in melanoma cells.

Fig. 4

(A) prediction of a putative miR-612 responsive site in the 3′-UTR of Espin based on the TargetScan algorithm (http://www.targetscan.org/vert_71/). (B) Luciferase reporter assay showing that miR-612 overexpression significantly suppressed the expression of the reporter gene with wild-type Espin 3′-UTR reporters. (C) Western blot analysis of ESPIN protein levels in melanoma cells transfected with vector or miR-612-expressing plasmids. *P < 0.05 relative to vector-transfected cells. (D) Pearson correlation analysis was performed to determine the correlation between miR-612 and Espin protein expression in 75 cases of melanoma tissues.