TABLE 3.
Device | Virus | Operator 1 |
Operator 2 |
Operator 3 |
Inter-CV (%) | |||
---|---|---|---|---|---|---|---|---|
CT (mean ± SD) | Intra-CV (%) | CT (mean ± SD) | Intra-CV (%) | CT (mean ± SD) | Intra-CV (%) | |||
Aries | FluA | 24.0 ± 0.80 | 3.3 | 24.3 ± 0.46 | 1.9 | 23.9 ± 0.35 | 1.5 | 2.1 |
FluB | 26.8 ± 0.14 | 0.5 | 26.7 ± 0.14 | 0.5 | 27.3 ± 0.40 | 1.5 | 1.9 | |
RSV | 24.8 ± 0.72 | 2.9 | 25.2 ± 0.82 | 3.3 | 25.1 ± 0.27 | 1.1 | 2.2 | |
Xpressb | FluA1 | 19.4 ± 0.27 | 1.4 | 19.0 ± 0.38 | 2.0 | 18.3 ± 0.27 | 1.5 | 2.9 |
FluA2 | 22.3 ± 0.50 | 2.3 | 21.8 ± 0.51 | 2.4 | 21.6 ± 0.78 | 3.6 | 2.7 | |
FluB | 20.8 ± 0.93 | 4.5 | 20.4 ± 0.35 | 1.7 | 20.7 ± 0.21 | 1.0 | 2.6 | |
RSV | 21.5 ± 0.15 | 0.7 | 21.3 ± 0.17 | 0.8 | 21.0 ± 1.28 | 6.1 | 3.2 |
CT, threshold cycle; CV, coefficient of variation.
Two channels (FluA1 and FluA2) are used to detect most influenza virus A strains.