Table 1. Primers and positive control strains used for multiplex PCR for detection of mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 genes.

Primer name	Sequence (5’-3’)	Target gene	Size (bp)	Positive control strain	Reference
mcr1_320bp_fw	AGTCCGTTTGTTCTTGTGGC	mcr-1	320	Escherichia coli 2012–60–1176–27 [22]	This study
mcr1_320bp_rev	AGATCCTTGGTCTCGGCTTG	mcr-1	320	Escherichia coli 2012–60–1176–27 [22]	This study
mcr2_700bp_fw	CAAGTGTGTTGGTCGCAGTT	mcr-2	715	E. coli KP37 [11]	This study
mcr2_700bp_rev	TCTAGCCCGACAAGCATACC	mcr-2	715	E. coli KP37 [11]	This study
mcr3_900bp_fw	AAATAAAAATTGTTCCGCTTATG	mcr-3	929	E. coli 2013-SQ352	This study
mcr3_900bp_rev	AATGGAGATCCCCGTTTTT	mcr-3	929	E. coli 2013-SQ352	This study
mcr4_1100bp_fw	TCACTTTCATCACTGCGTTG	mcr-4	1,116	E. coli DH5α [13]	This study
mcr4_1100bp_rev	TTGGTCCATGACTACCAATG	mcr-4	1,116	E. coli DH5α [13]	This study
MCR5_fw	ATGCGGTTGTCTGCATTTATC	mcr-5	1,644	Salmonella 13-SA01718 [14]	[14]
MCR5_rev	TCATTGTGGTTGTCCTTTTCTG	mcr-5	1,644	Salmonella 13-SA01718 [14]	[14]