Table 1. PCR assays used to detect or quantify all enteric viruses studied, Clermont-Ferrand, 2014–2015.
| Virus | Real-time assay | Detection | Limit of detection |
|---|---|---|---|
| Adenovirus | RealStar Adenovirus PCR Kit 1.0 (Altona) | Quantitative | 1.09 copies/μL |
| Enterovirus | In-house assay [11] | Quantitative | 6 copies/µL |
| Enterovirus@ceeramTools (Ceeram) | Qualitative | NA | |
| Parechovirus | Parechovirus r-gene (bioMérieux) | Qualitative | 0.62 TCID50/mL (PeV1) 9.96 TCID50/mL (PeV2) |
| Rotavirus | RealStar Rotavirus PCR Kit 1.0 (Altona) | Qualitative | 1.2 copies/μL |
| Norovirus (GI, GII)a | RealStar Norovirus PCR Kit 2.0 (Altona) | Qualitative | GI: 0.33 copies/μL GII: 0.25 copies/μL |
| Hepatitis A virus | RealStar HAV RT-PCR Kit 1.0 (Altona) | Qualitative | 0.46 IU/μL |
| Hepatitis E virus | RealStar HEV RT-PCR Kit 1.0 (Altona) | Qualitative | 0.31 IU/μL |
NA: not available from the manufacturer.
a Test allowing differentiation between genogroup I and genogroup II.