Figure 3.

Eosinophil detection in cotton rat BAL cytology is improved by using Hansel stain rather than Wright-Giemsa. In cytospin preparations of cells from cotton rat BAL, eosinophils (black arrowheads) are clearly distinguishable from neutrophils (white arrowheads) by using either staining method. However, (A) Wright–Giemsa also stains neutrophil cytoplasmic granules red. (B) In contrast, Hansel stain does not stain the cytoplasmic granules of cotton rat neutrophils as intensely as eosinophils, thereby enhancing the contrast between eosinophils and neutrophils for cell differentiation. Magnification, 100×.