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. 2017 Dec 19;22(3):1489–1500. doi: 10.1111/jcmm.13412

Figure 4.

Figure 4

Inhibiting miR‐126 promoted Spred1 expression in endothelial progenitor cells (EPCs). Bone marrow‐derived EPCs were transfected with 100 nM antagomiR‐126 after cultured for 7 days. And antagomiR negative control (Neg Ctrl) was used as control group. The expression of miR‐126 and mRNA of Spred1 was tested by quantitative real‐time PCR after 24 hrs transfection. Relative expression of miR‐126 (A). Quantitative analysis of relative expression of Spred1 mRNA (B). The protein of sprouty‐related EVH1 domain containing 1 (SPRED1) was tested by Western blotting after 48 hrs transfection. Examples of SPRED1 protein band in Western blotting (C). Quantitative analysis of relative expression of SPRED1 protein (D). *P < 0.05, versus negative control (n = 3).