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. 2017 Dec 19;22(3):1489–1500. doi: 10.1111/jcmm.13412

Figure 6.

Figure 6

1‐trifluoromethoxyphenyl‐3‐(1‐propionylpiperidine‐4‐yl) urea (TPPU) activated the protein kinase B/glycogen synthase kinase 3β (AKT/GSK3β) signalling pathway. Western blot testing of relative expression of AKT, p‐AKT, GSK3β and p‐GSK3β in EPCs from TPPU‐treated myocardial infarction (MI) ET mice. Examples of protein bands are shown (A). Relative expression of p‐AKT (B) and p‐GSK3β (C). Western blot testing of relative expression of AKT, p‐AKT, GSK3β and p‐GSK3β in TPPU‐treated and TPPU plus 14,15‐EEZE‐treated EPCs from MI mice. Examples of protein bands are shown (D). Relative expression of p‐AKT (E) and p‐GSK3β (F). *< 0.05, versus 0 mg/l TPPU‐treated group. < 0.05, versus 0 μM TPPU group. # < 0.05, versus 10 μM TPPU group (n = 3).