Figure 1.

Compared to other blood‐derived stem cell populations, CLP‐CMCs have a distinctive stemness signature. Morphological study and stemness characterization of human CLP‐CMCs. (A) Optical microscopy image of CLP‐MB and CLP‐CMC sprouted cells at early and late‐phases during 21 days of in vitro culturing. Scale bar: 25 μm. (B) Calculation of doubling population time (DPT) over a total of 16 divisions. (C) Gene expression analysis of pluripotency markers by quantitative PCR in cells grown in proliferative medium. The comparative CT method (2^−ΔCt ± S.D) was used to quantify the gene expression level. HPRT was considered as housekeeping gene.