Figure 2.

Hypo‐expression of ODF family proteins in asthenozoospermia is associated with axoneme instability. (A) Representative Western blots for protein levels of ODF1‐4, acetylated tubulin and total α‐tubulin in sperm from normozoospermic and asthenozoospermic samples. Prm1 protein was assayed as a loading control. (B) Quantification of band intensity for ODF1‐4, acetylated tubulin and total α‐tubulin in panel A. The data are presented as the means ± S.E.M. Student's t‐test was used for the statistical analysis. Statistical significance is expressed relative to the normozoospermic samples. *P < 0.05; ***P < 0.001. (C) Representative Western blots show the expression of Odf family proteins and acetylated and total α‐tubulin in four species (humans, mice, guinea pigs and rats). (D) The expression of Odf family proteins and acetylated and total α‐tubulin in four species. (E) The expression levels of acetylation of α‐tubulin in four species. The data are presented as the means ± S.E.M. Statistical significance is expressed relative to the human samples.