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. 2018 Jan 5;22(3):1816–1825. doi: 10.1111/jcmm.13464

Figure 6.

Figure 6

H3 relaxin inhibited P2X7R‐mediated NLRP3 inflammasome activation (A) The protein expression of NLRP3 inflammasome markers (NLRP3, cleaved caspase‐1, IL‐1β and IL‐18) was analysed by Western blot. (B) The protein levels of NLRP3 were normalized to β‐actin (NLRP3/β‐actin). (C) The protein levels of cleaved caspase‐1 were normalized to β‐actin (cleaved caspase‐1/β‐actin). (D) The protein levels of IL‐1β were normalized to β‐actin (IL‐1β/β‐actin). (E) The protein levels of IL‐18 were normalized to β‐actin (IL‐18/β‐actin). (F) The protein levels of IL‐1β in cell culture media. (G) The protein levels of IL‐18 in cell culture media. Data are the means ± S.D., and each measurement carried out six times. *P < 0.05 versus control, **P < 0.01 versus control, # P < 0.05 versus HG, ## P < 0.01 versus HG, & P < 0.05 versus HG+BzATP, && P < 0.01 versus HG+BzATP.