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. 2017 Dec 20;22(3):1614–1626. doi: 10.1111/jcmm.13436

Figure 4.

Figure 4

The activation of the JNK pathway is responsible for PUMA up‐regulation and apoptosis induced by ATO combined with Dasatinib. (A) JNK, p‐JNK, p‐ATF‐2 and p‐Jun were detected by Western blot after ATO and/or Dasatinib treatment for 24 hrs. (B) SUP‐B15 cells were stably transfected with NC, JNK1‐a2‐M or JNK1‐b1‐M. Stably transfected cells were treated with ATO (2 μΜ) combined with Dasatinib (40 nM) for 24 hrs. The expressions of JNK and p‐JNK were detected by Western blot. (C) Stably transfected cells were treated with ATO (2 μΜ) combined with Dasatinib (40 nM) for 48 hrs, and apoptosis was measured by flow cytometry. (D) Stably transfected cells were treated with ATO (2 μΜ) combined with Dasatinib (40 nM) for 24 hrs. The expressions of PUMA, caspase‐9, 3 and PARP were detected by Western blot. #JNK1‐a2‐M; ##JNK1‐b1‐M. Each bar represented the mean ± S.E.M, n = 3. ***P < 0.001 versus NC (A+D) group.