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. 2018 Jan 15;22(3):1944–1956. doi: 10.1111/jcmm.13480

Figure 1.

Figure 1

Visualization of ZIP8, ZIP14 and ZnT8 localizations in H9c2 cells. Localizations either to the S(E)R or to the sarcolemma/plasma membrane of ZIP8 (A), ZIP14 (B) and ZnT8 (C) were visualized using confocal micrographs in H9c2 cells. To examine the subcellular localizations of these transporters to S(E)R, H9c2 cells were transfected with ER‐resident discosoma red fluorescent protein (dsRed‐ER) to label ER or incubated with PMCA antibody to label sarcolemma/plasma membrane and with DAPI (blue) as well. Cells were incubated only with secondary antibodies and mounted with mounting medium contains DAPI (blue), and then images were merged.