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. 2018 Jan 15;22(3):1944–1956. doi: 10.1111/jcmm.13480

Figure 2.

Figure 2

Expression levels of ZIP8, ZIP14 and ZnT8 in heart tissue homogenates and doxorubicin‐treated H9c2 cells. Representative Western blotting bands for protein expression levels are given left part of figures in (A) and (B). The densitometry analysis showing the proteins of ZIP8, ZnT8 and ZIP14 is expressed at 50 kD, 41 kD and 50 kD, respectively, in doxorubicin‐treated H9c2 cells (DOXO group) with respect to untreated cells (CON group) (A) and underwent orthotopic heart transplantation with end‐stage heart failure (HF group) with respect to unsuitable for cardiac transplantation patients (CON group) (B) GAPDH at 37 kD as reference protein. Bars represent mean (±S.E.M.) for each group. Number of human samples, n = 3 for HF group and n = 2 for CON group. All measurements with double assays in each sample from each group for each type of measurement. Significance level accepted at *P < 0.05 versus CON.