Table 3.
Primer sequences for GP73, TGF‐β1, Smad2, E‐cadherin, N‐cadherin, vimentin, WT1 and GAPDH genes
| Gene | Forward | Reverse |
|---|---|---|
| GP73 | GTGCTGGTGCCAGCCTGTTA | AGTGCTCTAGGCCATTGATTGATTG |
| TGF‐β1 | AACCCACAACGAAATCTA | TGAGGTATCGCCAGGAAT |
| Smad2 | CGTCCATCTTGCCATTCACG | CTCAAGCTCATCTAATCGTCCTG |
| E‐cadherin | AACGCATTGCCACATACAC | GAGCACCTTCCATGACAGAC |
| N‐ cadherin | TGCGCGTGAAGGTTTGCCAGT | TGGCGTTCTTTATCCCGGCGT |
| Vimentin | ACAGGCTTTAGCGAGTTATT | GGGCTCCTAGCGGTTTAG |
| WT1 | CAAGGACTGCGAGAGAAGGTTT | TGGTGTGGGTCTTCAGATGGT |
| GAPDH | CCATGGAGAAGGCTGGGG | CAAAGTTGTCAT GGATGACC |
GP73, Golgi membrane protein 73; TGF‐β1, transforming growth factor‐β1; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; WT1, Wilms' tumour gene 1.