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. 2018 Jan 12;22(3):1909–1922. doi: 10.1111/jcmm.13475

Figure 5.

Figure 5

Hyper‐acetylation, but not HDAC5, contributes to chemo‐resistance in PCa. (A) Western blot analyses of HDAC family protein expression differences in two cell lines. (B) Knock‐down HDAC5 by siRNA in PC3/Doc cells, cell proliferation assay was monitored by MTT assay in response to the different concentration of docetaxel for 48 hrs. (C) Analysis of the effect of 0.4 μM TSA on the HDAC family by Western blot analysis. (D) The total HDAC activity was measured, in resistant cells compared to parent cells. ***P < 0.01 compared with PC3 cells. (E) The total HDAC activity was measured with or without 0.4 μM TSA treatment for 9 hrs in PC3/Doc cells. ***P < 0.001 compared with the negative control. (F) Western blot analyses of the acetylation status of the total protein, p300 and MOF in PC3 and PC3/Doc cells.