Figure 6.

TSA increases histone acetylation and exacerbates chemo‐resistant death of cells that are hyper‐acetylated. (A) Analysis of the acetylation change of protein treated with 0.4 μM TSA in PC3/Doc cells. (B) Analysis of the acetyl‐CoA level of different organelles in PC3 and PC3/Doc cells with or without 0.4 μM TSA treatment for 9 hrs. (C) The acetyl‐CoA‐related candidate genes were evaluated by quantitative PCR assays. (D) TSA treatment results increase histone acetylation. (E) TSA‐induced increase in H4K16 acetylation at the HSPA5 and ATF4 proximal promoter. The primer sets, shown in the Materials and methods. H4K16 acetylation level at the GAPDH promoter was used as a control. The data are represented as the averages ± S.D. for at least three samples. *P < 0.05 compared with the negative control. (F) Western blot analyses of the acetylation status of the total protein and proteins associated with ER stress in different treatment groups.