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. 2018 Jan 25;22(3):1864–1872. doi: 10.1111/jcmm.13470

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© 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Sox6 repressed Twist1 expression by recruiting HDAC1 (A) The pGL3.0 vector containing wild‐type (wt) or mutated Twist1 promoter was transfected into 293T cells with or without Sox6 expression. Thirty‐six hours after transfection, luciferase activity was detected using a dual‐luciferase reporter assay system and normalized to Renilla activity. (B) ChIP assay showed binding of Sox6 to the Twist1 promoter. (C) Western blot assay of Twist1 expression in pancreatic cancer cells overexpressing Sox6. (D) ChIP assay showed that HDAC1 binds to the Twist1 promoter in PC cells with or without Sox6 overexpression. (E) mRNA expression of E‐cadherin and N‐cadherin in Panc‐1 cells overexpressing Sox6 in the presence or absence of Twist1 overexpression. (F) Correlation of Sox6 and Twist1 expression in PC tissues. *P < 0.05; **P < 0.01.