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. 2017 Dec 20;22(3):1527–1537. doi: 10.1111/jcmm.13420

Figure 3.

Figure 3

DANCR and miR‐496 directly target each other. (A) Binding site of miR‐496 and DANCR as predicted by Starbase v2.0. (B) Relative luciferase activity in HEK‐293T cells transfected with miR‐496 mimic or mimic‐NC and cotransfected with the control vector, pMIR‐REPOR‐DANCR‐wt or pMIR‐REPOR‐DANCR‐mut. (C) RIP assays were conducted to evaluate the amount of DANCR bound to Ago2. (D) Mount of DANCR bound to biotin‐labelled miR‐496 mimic or biotin‐labelled negative control. RNA pull‐down assays were performed after 24 hrs of transfection. **P < 0.05.