Skip to main content
. 2017 Nov 29;22(3):1684–1695. doi: 10.1111/jcmm.13446

Figure 5.

Figure 5

FHL2 is an endogenous regulator for β‐catenin nuclear translocation. (A and B) Ectopic expression of FHL2 promotes β‐catenin translocation into the nuclei. NRK‐52E cells were transiently transfected with either FHL2 expression vector (pFHL2) or empty vector (pcDNA3). (C and D) Knockdown of endogenous FHL2 promotes β‐catenin translocation out of the nuclei. NRK‐52E cells were transfected with either control or FHL2 siRNA. Nuclear and cytoplasmic proteins from whole cell lysates were prepared and immunoblotted with antibodies against β‐catenin, TBP or GAPDH, respectively (A–D). (E) Forced expression of FHL2 induces β‐catenin activation in tubular epithelial cells. NRK‐52E cells were transiently transfected with either pFHL2 or pcDNA3, and the whole cell lysates were immunoblotted with antibodies against DDK, active β‐catenin, β‐catenin or GAPDH, respectively. (F) Knockdown of endogenous FHL2 decreases β‐catenin activation. NRK‐52E cells were transfected with either control or FHL2 siRNA, and the whole cell lysates were immunoblotted with antibodies against FHL2, phosphorylated β‐catenin (Ser33/37/Thr41), β‐catenin or GAPDH, respectively.