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. 2017 Dec 26;22(3):1583–1600. doi: 10.1111/jcmm.13434

Figure 5.

Figure 5

Mtp alleviates oxidative stress‐mediated mitochondrial dysfunction in BM‐EPCs. (A–C) Fluorescence staining results of mitochondrial membrane potential by rhodamine 123 and ROS production by ROS probe DHE. BM‐EPCs were treated with 100 μM Mtp for 48 hrs and TBHP for 3 hrs and then labelled with fluorescent dyes rhodamine 123, DHE and Hoechst 33258. Representative images were taken from stained BM‐EPCs of indicated groups (scale bar: 30 μm). MMP significantly increased and ROS products decreased in Mtp and/or TBHP‐treated BM‐EPCs; (D–F) immunofluorescence staining results of Bax and Bcl‐2 in Mtp and/or TBHP‐treated BM‐EPCs (scale bar: 50, 50 μm); (G–I) Western blot analysis of protein expression of Bax, Bcl‐2, cytochrome c, caspase 9 in BM‐EPCs treated with 100 μM Mtp for 48 hrs and TBHP for 3 hrs. Pre‐treatment with Mtp evidently decreased the release of pro‐apoptotic proteins. The densitometric analysis of all Western blot bands was normalized to the total proteins or GAPDH. n = 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.005, and ****P < 0.001 versus the indicated group.