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. 2017 Dec 26;22(3):1583–1600. doi: 10.1111/jcmm.13434

Figure 6.

Figure 6

Mtp provides cellular protection against apoptosis in BM‐EPCs via mTOR signalling pathways. (A, B) Immunofluorescence staining images and intensity of LC3‐positive autophagic vesicles (scale bar: 50 μm). LC3 autophagic vesicles were significantly decreased by Mtp pre‐treatment; (C–H) protein levels of p‐mTOR, p‐p70S6K, p‐4EBP1, SQSTM1/P62, Beclin‐1 and LC3‐II, cleaved‐caspase 3, Bax, Bcl‐2, cytochrome c, caspase 9 in BM‐EPCs treated with 100 nM rapamycin for 2 hrs, 100 μM Mtp for 48 hrs and TBHP for 3 hrs; (I) cell viability results by CCK‐8 test of BM‐EPCs treated with 100 nM rapamycin for 2 hrs, 100 μM Mtp for 48 hrs and TBHP for 3 hrs. Cell viability was evidently increased by the Mtp pre‐treatment. The densitometric analysis of all Western blot bands was normalized to the total proteins or GAPDH. n = 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.005, and ****P < 0.001 versus the indicated group.