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. 2017 Dec 26;22(3):1583–1600. doi: 10.1111/jcmm.13434

Figure 7.

Figure 7

Suppression of AMPK by Mtp attenuates oxidative stress‐mediated cell apoptosis and autophagy. (A, B) Western blot analysis of expression of p‐AMPK in different doses of Mtp treatment. BM‐EPCs were treated with 1, 10 and 100 μM Mtp. The protein expression of p‐AMPK was decreased after Mtp treatment; (C, D) Western blot analysis of expression of p‐AMPK after Mtp pre‐treatment. Cells were pre‐treated with 100 μM Mtp followed by TBHP stimulation. Mtp reduced p‐AMPK protein expression of BM‐EPCs induced by TBHP; (E–O) Western blot analysis of expression of p‐AMPK, p‐mTOR, cleaved‐caspase 3, Bax, Bcl‐2, caspase 9, SQSTM1/P62 and LC3‐II. Cells were pre‐treated with 5 μM compound C or 1 mM AICAR for 2 hrs followed by 100 μM Mtp for 48 hrs and incubated with TBHP for 3 hrs. (P) Cell Counting Kit‐8 (CCK‐8) results of BM‐EPCs were treated under the same conditions as above. Cell viability was significantly increased by Cpd C treatment. The densitometric analysis of all Western blot band intensities was normalized to the total proteins or GAPDH. n = 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.005, and ****P < 0.001 versus the indicated group.