Fig. 2.

Effects of miR-448 on IL-1β-induced inhibition of aggrecan and type II collagen mRNA expressions inchondrocytes. a Chondrocytes were transfected with miR-448 mimic or inhibitor. The level of miR-448 was determined using quantitative real-time PCR. Chondrocytes were treated with 5 ng/ml IL-1β for 24 h after transfection with miR-448 inhibitor b or mimic c. The mRNA levels of aggrecan and type II collagen were determined using quantitative real-time PCR. The data shown are means ± SEM, n = 4. **p < 0.01 vs. control; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. vehicle + IL-1β