Fig. 6.

Effects of miR-448 on osteoarthritis chondrocytes. Osteoarthritis chondrocytes were transfected with miR-448 mimic or inhibitor. a The protein level of matrilin-3 were determined using western blot. The mRNA levels of aggrecan (b) and type II collagen (c) were determined using quantitative real-time PCR. GAPDH was detected as a loading control. d The expression level of secreted MMP-13 protein was determined using ELISA. All data are presented as means ± SEM, n = 4. ^##p < 0.01 vs. miR-NC or anti-miR-NC