Table 2.
Problem | Reason | Solution |
---|---|---|
Poor germination and seedling growth | Used floaters Poor storage of seeds |
Use sinkers Use desiccants during storage at 4 °C |
Lack of induction of hairy roots |
Agrobacterium may lose virulence over the culturing process or become contaminated Seedling viability is too low Plants were not periodically watered |
Use fresh − 80 °C glycerol stock Grow new seedlings Water plants regularly |