Table 2.

Troubleshooting

Problem	Reason	Solution
Poor germination and seedling growth	Used floaters Poor storage of seeds	Use sinkers Use desiccants during storage at 4 °C
Lack of induction of hairy roots	Agrobacterium may lose virulence over the culturing process or become contaminated Seedling viability is too low Plants were not periodically watered	Use fresh − 80 °C glycerol stock Grow new seedlings Water plants regularly