Fig. 3.

Diabetes affects calbindin+ interneurons and impairs adult neurogenesis in the olfactory bulb. a Density of CB+ interneurons. b-c representative microphotographs of CB+ staining in the MOB of non-diabetic Wistar versus T2D GK rats (n = 5–6). d Relative level of CB expression normalized against beta-actin and, e representative bands showing CB expression level in the MOB of non-diabetic vs. T2D rats (n = 4), (Western blot). f Density of immature DCX+ neurons and, g-h representative microphotographs of DCX+ staining in the MOB of Wistar vs. GK rats (n = 7) (black arrows indicate DCX+ cells). Two-tailed, unpaired t-test. The data are means ±S.E.M., * p < 0.05