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Primer sets used in this study
| Primer designation | Sequence (5ʹ → 3ʹ) | Step | Product length (bp) | Target region |
|---|---|---|---|---|
| HEV-s | TCGCGCATCACMTTYTTCCARAA | RT-PCR | 469 | ORF1 |
| HEV-as | GCCATGTTCCAGACDGTRTTCCA | |||
| HEV-fn | TGTTGCCCTGTTTGGCCCCTGGTTTAG | Nested RT-PCR |
254 | |
| HEV-rn | CCAGGCTCACCRGARTGYTTCTTCCA | |||
| 3156F | AATTATGCYCAGTAYCGRGTTG | RT-PCR | 731 | ORF2 |
| 3157R | CCCTTRTCYTGCTGMGCATTCTC | |||
| 3158Fn | GTWATGCTYTGCATWCATGGCT | Nested RT-PCR |
348 | |
| 3159Rn | AGCCGACGAAATCAATTCTGTC |
