Fig. 3.

Culture media modifies IL-1β-induced transcription factor expression in human brain pericytes. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. For the final 1 h (a) or 24 h (b) 10 ng/mL IL-1β or vehicle was added. Pericytes were fixed and immunostained for the transcription factors NF-kB p65 (a) or C/EBPδ (b). Nuclei were counterstained with Hoechst. The percentage of cells displaying nuclear NF-kB p65 (c) or scored positive for C/EBPδ (d) was determined by automated image analysis. RNA was extracted from pericytes treated with 10 ng/mL IL-1β or vehicle (0.0001% BSA in PBS) for the final 24 h of a 7 day culture and the gene expression of CEBPD determined by qRT-PCR (e). Data are displayed as mean ± SEM of three independent experiments. NS = p > 0.05; **p < 0.01; ***p < 0.001 as designated or compared to vehicle control in respective media (Two-way ANOVA). Scale bar = 50 µm