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. Author manuscript; available in PMC: 2018 Jun 3.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Apr 13;487(3):545–551. doi: 10.1016/j.bbrc.2017.04.067

Figure 1. VWA8 protein is expressed widely and VWA8 protein and mRNA are elevated in response to high fat feeding.

Figure 1

A. Lysates of indicated mouse tissues were subjected to immunoblot analysis using an antibody directed against VWA8a, with tubulin as a loading control. B. Livers from six C57BL/6J mice fed a low fat diet for 10 weeks (L), and six C57BL/6J mice fed a high fat diet were homogenized, and lysates were subjected to immunoblot analysis with anti-VWA8a antibody or β-actin (control). C. Total mRNA from the same two groups (LFD, low fat diet; HFD, high fat diet) of 6 mice each was subjected to Q-rtPCR analysis of mRNA for VWA8a and VWA8b. Values for individual mRNA levels were expressed relative to the average for the low fat diet group (*P<0.05 vs. 10% LFD).