Fig. 3. Exogenous Cu enhances myogenic differentiation. (A) Representative light micrographs of differentiating myoblasts the absence of insulin (top panel, –Ins, –Cu) and or in the absence of insulin but supplemented with 30 μM CuSO4 (bottom, –Ins, +Cu). (B) Steady state mRNA levels of the myogenic Myog, MyhII, Acta1, and Ckm in primary myoblasts cultured in the same conditions as indicate in panel (A). Control depicts the same control values used in Fig. 2. Data represent the mean of at least four independent biological replicates ±SD. **P < 0.01.
