Fig. 4. Cu chelation impairs primary myoblast growth. (A) Representative light micrographs of proliferating myoblasts grown in normal growth medium (control) or in the presence of Cu (100 μM CuSO4), TEPA (100 μM TEPA), and Cu/TEPA (100 μM TEPA and CuSO4). (B) Cell counting assay of primary myoblasts grown under the same culture conditions as in panel A. Data represent the average of three independent experiments ±SD. (C) Steady state Pax7 mRNA levels in myoblasts cultured 48 h in the same growth conditions as in panel (A). All data represent the average of at least three independent biological experiments ± SD; **P < 0.01.
