Fig. 5. ATP7A and ATP7B expression in the myogenic lineage. (A) Atp7a expression is induced upon initiation of myogenic differentiation. Steady-state Atp7a mRNA levels in proliferating and differentiating primary myoblasts as determined by qRT-PCR. (B) Atp7b is constitutively expressed in proliferating and differentiating primary myoblasts. Steady state Atp7b mRNA levels in proliferating and differentiating primary myoblasts as determined by qRT-PCR. (C) ATP7A and ATP7B levels shown by representative Western blot and densitometric quantification of ATP7A (D) and ATP7B (E) bands in proliferating and differentiating primary myoblasts. GAPDH was used as loading control. All data represent the average of three independent biological experiments ± SD; **P < 0.01.