Fig. 7. Atp7a mRNA is regulated at the post-transcriptional level during myogenic differentiation. (A) Schematic of Atp7a 3′ UTR showing putative PAS, GREs, and ARE. (B) Schematic of qRT-PCR strategy for quantifying PAS utilization in Atp7a mRNA. (C) Altered PAS utilization in Atp7a during myogenic differentiation as determined by qRT-PCR assay indicated in panel (B). (D) Atp7a mRNA in proliferating myoblasts is unstable as determined by actinomycin D treatment followed by qRT-PCR. Stability of Myc mRNA was used as a positive control. (E) Atp7a mRNA in differentiated myotubes is stable as determined by actinomycin D treatment followed by qRT-PCR. Stability of Myc mRNA was used as a positive control. All data represent the average of three independent biological experiments ± SE; *P < 0.05, **P < 0.01, ***P < 0.001.