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. Author manuscript; available in PMC: 2018 Feb 23.
Published in final edited form as: Nat Neurosci. 2017 Feb 6;20(3):396–405. doi: 10.1038/nn.4493

Figure 2.

Figure 2

Astrocyte populations demonstrate diverse developmental functions. (a–d) Plots showing the mean and standard error of FACS analysis for each subpopulation in the cortex across postnatal time points P1 (a), P7 (b), P14 (c) and P28 (d); Data are mean ± s.e.m. For each time point, data were derived from 4 independent FACS experiments; for each experiment, the cortices from 4 mice were pooled and analyzed (associated statistical analysis in Supplementary Fig. 3). (e) Code between population nomenclature (A–E) and cell surface marker combination. (f–k) Representative images for BrdU staining (f,g) or Transwell assays (h,i) from the acute culture of FACS-isolated populations A (f,h) and C (g,i) and quantification for BrdU staining (j) or migration through the matrix in the Transwell assays (k). DAPI was used to stain nuclei. Data are mean ± s.e.m. Quantification was from four independent cell cultures derived from four independent FACS isolation procedures; each experiment was performed in triplicate. *P = 5.22 × 10−4, **P = 2.29 × 10−5, ***P = 0.02784 and ****P = 0.00391; F2,11 = 41.95 (j) and F2,11 = 10.62 (k); by one-way analysis of variance (ANOVA) and followed by Tukey’s test for between-group comparisons. Scale bars, 100 μm (f,g) and 200 μm (h,i).