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. Author manuscript; available in PMC: 2018 Feb 23.
Published in final edited form as: Mol Cell. 2016 Oct 20;64(3):455–466. doi: 10.1016/j.molcel.2016.09.002

Figure 3. The Tail Domain Is Required for Mediator-UAS Interactions but Dispensable for Mediator to Associate with Core Promoters.

Figure 3

(A) Med7 (green) occupancy at the SAGA-dominated gene PMA1 (left) and the TFIID-dominated gene SCW4 (right) in WT and med3Δ/med15Δ (TailΔ) cells, both in a KIN28 WT and kin28as backgrounds. All samples were treated with NAPP1. TFIIB (tan) is shown as a placeholder for core promoters. Vertical dashed lines indicate the position of the UAS and TSS.

(B) Heatmap representation of Med7 occupancy in WT (left) and med3Δ/med15Δ (TailΔ, right) cells around UAS regions (n = 498, see Experimental Procedures) sorted by decreasing Mediator occupancy.

(C) Heatmap representation of Med7 occupancy in kin28as (WT, left) and kin28as/med3Δ/med15Δ (TailΔ, right) cells around the TSS of genes associated to the UAS regions used in (B). Traces at the bottom of each heatmap from (B) and (C) show the average occupancy across these loci.

(D) Cumulative plot of the change in Med7 occupancy in WT versus med3Δ/med15Δ (TailΔ) cells at UAS (solid trace) and at core promoters (CP, dashed traces). UAS and core promoter traces were calculated from experiments performed in KIN28 WT and kin28as cells treated with NAPP1 respectively, using the same loci as in (B) and (C).

See also Figure S3.