(A) Cumulative plot of the change in TFIIB promoter occupancy in med18-AA cells after a 90 min treatment with rapamycin (solid trace) and in med3Δ/med15Δ cells (TailΔ, dashed trace), relative to their respective WT. All genes with a TFIIB log2 ratio > 2 were used in this analysis.
(B) Cumulative plot of the change in TFIIB promoter occupancy in med3Δ/med15Δ cells (TailΔ) cells, relative to WT, for the SAGA-dominated (red) and TFIID-dominated (blue) genes with a TFIIB log2 ratio > 2.
(C) Cumulative plot of the change in TFIIB promoter occupancy in med18-AA cells after a 90 min treatment with rapamycin, relative to WT, for the SAGA-dominated (red) and TFIID-dominated (blue) genes with a TFIIB log2 ratio > 2 (high TFIIB, solid traces) or for genes having equivalent TFIIB levels between the SAGA- and TFIID-dominated gene groups (equivalent TFIIB, dashed traces).
(D) Distribution of TFIIB occupancy at the promoter of SAGA-dominated genes (red) and TFIID-dominated genes (blue).
See also Figure S4.