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. 2018 Feb 20;11:891–901. doi: 10.2147/OTT.S154754

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© 2018 Shen et al. This work is published and licensed by Dove Medical Press Limited

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CDCA5 knockdown suppresses HCC cell growth.

Notes: (A) CDCA5 expression in Huh7 cells after 72-h transfection with lentiviruses encoding three differing CDCA5 specific sh-RNAs or the control were analyzed using Western blotting. GAPDH was used as loading control. (B, C) Growth of transfected Huh7 cells was monitored using a microscope, and images were taken using a phase contrast fluorescence microscope in light (B) or fluorescence (C) models for expression of green fluorescent protein at a magnification of 200×. (D) Cell growth was determined by cell counting using a Countstar Automated Cell Counter (*P<0.05).

Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HCC, hepatocellular carcinoma; sh-Ctrl, control.