Figure 4.

CDCA5 knockdown reduces viability and survival and induces cell cycle arrest of HCC cells.

Notes: (A) Cell counting kit-8 assays were performed to determine Huh-7 cell viability following transfection with the shRNA encoding lentiviruses above, and viability was normalized to the absorbance at 450 nm of Day 1 for each group; *, #, and Δ represent sh-CDCA5-1, sh-CDCA5-2, and sh-CDCA5-3 versus sh-Ctrl, respectively; P<0.05. (B) Colony formation assays were used to determine cell survival after 72-h transfection; 800 cells from each group were reseeded into 12-well plates and were cultured for 8–10 days. Colonies were stained with crystal violet, and survival rates were normalized to the numbers of colonies in the sh-Ctrl (100%) group, *P<0.05 versus sh-Ctrl. (C) After transfection with the three different sh-CDCA5 or sh-Ctrl lentiviruses for 72 h, Huh7 cells were stained with propidium iodide solution and were analyzed using flow cytometry. Cell cycle distributions (upper panel) and percentages of G2/M phase cells (lower panel) are shown; *P<0.05 versus sh-Ctrl.

Abbreviations: HCC, hepatocellular carcinoma; sh-Ctrl, control.