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. 2018 Feb 23;8:3568. doi: 10.1038/s41598-018-21656-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hippocampal Kv1.1 mRNA (KCNA1) levels are not altered in adult NS-Pten KO mice. Relative quantification of Pten and Kv1.1 (KCNA1) mRNA expression using RT-qPCR analysis on total hippocampal RNA isolated from 8 week-old WT and NS-Pten KO mice are shown. Pten mRNA levels were significantly decreased in NS-Pten KO compared to WT mice. No significant changes in Kv1.1. mRNA levels were observed between NS-Pten KO and WT mice. β-actin and GAPDH expression were used as internal controls. n = 7–8 mice per group; ***p < 0.001 by Student’s t-test; errors bars are ± SEM.