Figure 7.

Carnosol inhibited UVB-induced NF-κB activation. Cells were exposed to UVB radiation (50 mJ/cm²) in the presence or absence of carnosol. Cells were lysed at indicated time point and protein levels were determined by western blot analysis. (A) Western blot for IκB protein level in HaCaT cells at 0, 2, 4 hours post UVB radiation at 1, 10 or 20 μM carnosol treatment. (B) Western blot analysis for IκB protein level in HaCaT cells at 6 hours post UVB radiation in the presence or absence of carnosol (10 or 20 μM). (C) Western blot for S276 site phosphorylation of NF-κB in HaCaT cells at 6 hours post UVB radiation with 10 or 20 μM carnosol treatment. See supplement material for full-length western blot figure. (D) EMSA assay and its statistical analysis for HaCaT cells at 0, 2, 4, 6 hours post UVB radiation in the presence or absence of carnosol (20 μM). **p < 0.01.