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. 2018 Feb 23;8:3561. doi: 10.1038/s41598-018-21446-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification the essential motif and site responsible for the self-interaction of V2. (a) Schematic representation of the motif and sites of TYLCV V2 based online prediction. (b) Schematic representation of the truncated mutants of V2 and yeast-two-hybrid analysis of the interactions with V2. (c) Identification of the interaction between the wide-type V2 and the site-directed mutants V2^G70A, V2^S71A, V2^K73A in a yeast two-hybrid assay.