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. 2018 Feb 23;8:3561. doi: 10.1038/s41598-018-21446-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Symptoms exhibited by N. benthamiana plants infected with PVX expressing V2 and V2^S71A. (a) The symptoms of N. benthamiana plants at 16 dpi. (b) Western blot analyses of V2 and V2^S71A expression with pGR-V2, pGR-V2^S71A and pGR. (c) The virus accumulation in plants infiltrated with pGR-V2, pGR-V2^S71A and pGR. (d) Sequencing analysis of V2 in plants infiltrated with pGR-V2 and pGR-V2^S71A.