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. 2018 Feb 23;9:796. doi: 10.1038/s41467-018-03185-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Graphene increases cell membrane cholesterol. a Sample images of Filipin staining. Scale bar, 100 μm. b Filipin fluorescence intensity in neuronal neurites (n_glass = 118 and n_graphene = 112 neurites, N = 3 batches, ***p < 0.001, Wilcoxin rank-sum test, Cohen’s d = 1.89). c Sample generalized polarization (GP) images. Scale bar, 20 μm. d Distributions of GP values over individual image pixels (n = 7 FOVs, N = 3 batches; p < 0.05, Kolmogorov–Smirnov test of the distributions of GP values, see 'Statistical analysis' section in Methods). Error bars are S.E.M.