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. 2018 Feb 23;8:3533. doi: 10.1038/s41598-018-21521-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dendritic morphology quantified with 3D reconstructions of Golgi impregnated neurons and software-assisted tracing of entire dendritic arbors of stained neurons. (a) Golgi Staining of rat hippocampus. stratum oriens, SO; pyramidal cell layer, PCL; stratum radiatum, SR; stratum lacunosum-moleculare, SL-M; dentate gyrus, DG; scale bars = 200 μm. (b) Golgi staining of CA1 pyramidal neurons, scale bars = 100 μm. (c) Imaris reconstruction of CA1 pyramidal neurons (16 neurons/8 rats for all groups). (d) Sholl analysis was applied to measure the dendritic complicity of reconstructed pyramidal neurons, which showed no differences in the numbers of intersections for all groups of GLE Pb-treated rodents. Data are expressed as mean ± SD.