Figure 2.

SHLI fails to elevate serum tIgE (A) and MMCP1 (B) levels in antibiotic-treated mice (n = 8). We fed mice autoclaved water with or without ampicillin (0.5 mg/mL), gentamicin (0.5 mg/mL), metronidazole (0.5 mg/mL), neomycin (0.5 mg/mL), and vancomycin (0.25 mg/mL) continuously via a water bottle. In the event of poor animal hydration, we supplemented control and antibiotic water with artificial sweetener. Five weeks later, the mice were sacrificed, and the serum tIgE and MMCP1 levels were measured by ELISA. ^*P < 0.05 and ^***P < 0.001.