Figure 3.

SHLI induces vasopermeability (Evans Blue extravasation) during the first intraplantar injection (n = 6). Fifteen minutes after induction of anesthesia (50 mg/kg of pentobarbital), mice were injected intravenously (i.v.) with 50 μL of 12.5 mg/mL Evans Blue. Five minutes later, 5 μL of the test substance (C48/80, SHLI, F1, or F2) was administered by intraplantar injection in the right paw and NS was administered in the left paw. Thirty minutes later, the mice were sacrificed and the paw tissue was collected, dried for 24 h at 50 °C, and weighed. Evans Blue was extracted by a 24 h incubation in formamide at 50 °C, and the OD value was read at 620 nm. ^**P < 0.01 and ^***P < 0.001.