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. 2018 Feb 23;8:3572. doi: 10.1038/s41598-018-21843-7

Figure 5.

Figure 5

SHLI-induced NA-IHRs is mediated by C5a. (A) C5a antagonist PMX53 can block F2-induced shock (n = 10). The mice were pretreated with propranolol (i.v., 35 μg/mouse) 10 min after intraperitoneally injected with SB290157 (a C3a antagonist, 30 mg/kg) or PMX53 (a C5a antagonist, 1 mg/kg). Twenty minutes later, the mice were challenged (i.p.) with F2 (16.5 mg/mouse). Thirty minutes later, the rectal temperature was measured. *P < 0.05 and **P < 0.01. (B) Representative images of Evans Blue extravasation of mouse paw induced by F2 and blocked by PMX53 (n = 6). Fifteen minutes after induction of anesthesia (50 mg/kg of pentobarbital), mice were intraplantarly injected with 10 μL of PMX53 (1 mg/mL). Twenty minutes later, the mice were injected (i.v.) with 50 μL of 12.5 mg/mL Evans Blue. Five minutes later, 5 μL of F2 (33 mg/mL), or NS was administered by intraplantar injection in the paw. Thirty minutes later, the mice were sacrificed and the paws were photographed. (C) Representative images of Evans Blue extravasation of mouse paw induced by SHLI (10 μL/paw) and blocked by PMX53 (n = 6).