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. 2018 Feb 23;8:3521. doi: 10.1038/s41598-018-21942-5

Figure 1.

Figure 1

SAHA, tumor necrosis factor alpha (TNF-α), and phorbo-12-myristate-13-acetate (PMA) induce NanoLuc activity in THP-1-NanoLuc clones. (a) Schematic structure of an HIV-1 vector designated pNLn-NanoLuc-Kp is shown. The vector has a frame-shift mutation at the KpnI site in the env gene to avoid expression of the viral envelop protein. Part of the nef gene is replaced with NanoLuc that functions as a reporter gene. The VSV-G pseudotyped virus was produced by co-transfection into HEK293FT cells with pNLn-NanoLuc-Kp and a VSV-G expression vector. After THP-1 cells were infected with the virus, the infected clones were obtained by a limiting dilution method. (b) #95 and (c) #225 THP-1-NanoLuc clone cells were stimulated with 10 μM SAHA, 10 ng/mL TNF-α, or 10 ng/mL PMA for 24 hours. The cells were harvested and lysed. NanoLuc activity of the cell lysate was determined. Each value is shown as a mean ± standard deviation (SD) for triplicate samples.